InfrafrontierGR/Phenotypos

The services provided include:

 

Histology

• Tissue fixation and paraffin coating/embedding 

• Microtome sectioning (serial/step sectioning of fixed wet tissue or whole embryo samples)

• Unstained and/or H&E stained paraffin sections 

• All special stain protocols can be performed

• Ability to cut 3-150 Micron paraffin sections

• bone processing including decalcification

 

Frozen Sections

• Cryostat sectioning

• H&E frozen sections 

• Additional special stain protocols

• Additional frozen sections

• Initial unstained frozen sections

 

Soft organ and tissue Sections (mildly- / non-fixed)

The vibrating blade microtome enables the user to:

• obtain high quality sections that retain viable cells on the section surface

• accurately cut tissue without freezing or embedding

• maintain more live cells, cell morphology as well as enzyme and cell activity within the sections

• obtain sections of 20-100 micrometers 24 well plates

 

Immunohistochemistry (IHC) and/or in situ hybridisation (ISH)

• Whole-mount in situ hybridization/immunohistochemistry of early to mid-gestation mouse embryos

• Section in situ hybridization (mRNA, microRNA), immunohistochemistry, and immunofluorescence

• Unstained sections on slides suitable for IHC

• IHC with commercially available/validated antibodies

• Validation/Optimization of IHC of new investigational antibodies