© 2014 INFRAFRONTIER.GR Project Management

Mutant Mouse Creation

Transgenic mice are produced upon request via state-of-the-art genome manipulation techniques. The services result in the generation of mice carrying an inserted cassette in their genome, obligatory or conditional knock-out as well as knock-in mutant mice.
 
BSRC Alexander Fleming’s Transgenic Facility, is a facility of reference with a great record of transgenic lines produced.  Despite the difficulty of the procedures, the percentage of successful projects is over 90%. The Facility is certified with ISO 9001:2000.
Pronuclear microinjection of DNA constructs

Archiving services are provided by the CryoUnit at BSRC “Alexander Fleming” which reassures the maintenance, revival and transportation of mutant strains through embryo rederivation, cryopreservation and recovery of mouse embryo and sperm.

Gene targeting of embryonic stem cells (Esc)

 

Transgenic mice are produced upon request via state-of-the-art genome manipulation techniques. The services result in the generation of mice carrying an inserted cassette in their genome, obligatory or conditional knock-out as well as knock-in mutant mice.

Blastocyst Injections of mutated Esc clones

Infrafrontier-GR partners aim to advance medical and biological research by providing the scientific community with standardised, high-quality phenotyping services. All partners have extensive experience and expertise with state-of-the-art equipment and follow closely the dynamic and developing set of procedures, continuously adapting to new techniques and needs of the research community. 

Contact Us

TEL.

+30 210 9656310

 

EMAIL

infrafrontier-GR_services@fleming.gr

PERSONNEL

Dimitris L. Kontoyiannis, Ph.D.

Scientist in Charge

 

Demetris Vassilakos, PhD.

Operations Manager – Gene Targeting Specialist

 

Konstantinos Bozonelos, M.Sc.

Cryopreservation Specialist

 

Alexia Giannakopoulou

Microinjection Specialist

 

Panagiotis Papadopoulos

Technician/Animal Caretaker