Mouse archiving and distribution
Archiving services are provided by the CryoUnit at BSRC “Alexander Fleming” which reassures the maintenance, revival and transportation of mutant strains through embryo rederivation, cryopreservation and recovery of mouse embryo and sperm.
The CryoUnit has been accredited as a member of the European Mouse Mutant Archive (EMMA) network. To date, more than 300 cryopreserved lines exist in the CryoBank among which transgenic, knockouts and CRE lines. These lines are actively revived and distributed, thus continuously ensuring the good quality of cryopreserved material.
All services provided are in accordance with EMMA standards and protocols.
Three fertile males are provided by the investigator, at the age of 8 to 12 weeks. Sperm collection is performed for every male individually, from the cauda epididymis.
The facility generates frozen stock of mouse lines in the form of embryos. The service is applied for either strains deposited to the European Mouse Mutant Archive (EMMA) network or not.
Our Facility generates live mice by performing surgical transfer of embryos recovered from a cryopreserved stock (kept into our CryoBank or imported) or from fresh embryos collected by us.
In vitro fertilization
IVF is a method of assisted reproduction, in which fresh or cryopreserved sperm is used to fertilize mature oocytes in a tissue culture dish, producing a large number of embryos in a short time.
The service is provided for revival of lines whose sperm has been cryopreserved into our CryoBank or others. Additionally, it can be used as a rederivation method in case of males with fertility problems.
Storage and distribution
All cryopreserved material is stored into our CryoBank in liquid nitrogen tanks. The maintenance, replenishment and distribution of all cryopreserved lines is our responsibility.