Trans-national Access call - October 2018
Context and aim of the call
Main objective of this INFRAFRONTIER2020 Trans-national Access call is to facilitate access for the wider biomedical research community to the unique infrastructure and scientific expertise of the participating INFRAFRONTIER mouse clinics, where mouse mutant lines can be tested through specialised metabolic- and behavioural phenotyping pipelines. Disturbed energy balance regulation results in obesity and in numerous related metabolic disorders such as type 2 diabetes mellitus. We offer specialised phenotyping pipelines comprising state-of-the-art test assays monitoring disease related parameters. In-depth behavioural phenotyping supports the elucidation of the molecular and genetic basis of behavioural impairments that are relevant for human neuropsychiatric disorders such as anxiety disorders, post-traumatic stress disorder, depression, schizophrenia, autism, attention-deficit hyperactivity disorder, Parkinson’s and Alzheimer’s Disease.
Access will be granted on the basis of scientific excellence and supports the development and in depth characterisation of mouse models for investigating gene function and human pathophysiology. INFRAFRONTIER will provide open access to all characterised disease models and phenotyping data
The EC Horizon 2020 funded INFRAFRONTIER2020 project (2017 – 2020) supports eligible customers with a free-of-charge mouse model phenotyping service implemented as a Trans-national Access activity supporting a total of 9 projects in this first call.
The access unit offered covers the specialised metabolic or behavioural phenotyping of a mouse mutant line, and the preparation of a comprehensive phenotyping report.
Participating mouse clinics are the German Mouse Clinic (GMC), the Czech Centre for Phenogenomics (CCP), and PHENOMIN-ICS.
Centre specific collaboration agreements will be established between applicants and the mouse clinics. Selected projects will be allocated to the mouse clinics based on scope of submitted project proposals.
Starting material for the phenotyping projects are either experimental cohorts (10-12 mutant and control mice, 10-15 weeks of age, acceptance depending on health certificates, GMC and PHENOMIN-ICS) or a breeding nucleus and then cohorts are bred by mouse clinics (CCP). Mouse mutants from various sources (transgenes, knockout mice, mutants from mutagenesis screens like ENU) and of different genetic backgrounds can be accepted.
The phenotyping pipelines to be used will be specialised metabolic- or behavioural phenotyping pipelines based on published IMPReSS SOPs (IMPReSS: http://www.mousephenotype.org/impress), or on local mouse clinic SOPs. Details of the specific mouse clinic specialised phenotyping pipelines are described on page 5 of the application form.
Support will be provided by the mouse clinics to analyse and interpret the phenotype data.
The analysed mouse models and the generated phenotype data will be made available to the scientific community. An optional grace period of up to 1 year for mouse resources and phenotype data may apply, with immediate release of mouse resources and data after expiry of the grace period. A phenotyping report with all phenotype data will be prepared, and data will also be uploaded onto a public phenotype database of the mouse clinics and / or onto the IMPC portal at http://www.mousephenotype.org. Mouse mutant lines will be deposited into the INFRAFRONTIER/EMMA repository for subsequent use by the scientific community, and will be distributed using the applicant’s institutional MTAs.
The access to the INFRAFRONTIER2020 phenotyping service is free of charge. However, the shipment cost of mouse mutant lines to the participating mouse clinics must be borne by the applicants.