The PHENOMIN-CIPHE mouse clinic provides access to its infrastructure and expertise for analysing mouse mutant lines through specialised functional immune-phenotyping by mass cytometry. To characterize the functional impact, on the immune system, of the invalidation or the overexpression of a gene of interest, cellular immune-phenotype profiling is required. This primary immunophenotyping screen, essentially based on an extracellular labelling, aims at quantifying the various cell populations present within a model by monitoring the expression of specific cell surface receptors in hematopoietic cells. This first approach describing cellular heterogeneity does not assess the effector functions of immune cells. To this end, a more functional secondary immune-phenotyping screen is required and offered in this call.

Access to the mouse clinics and their specialised phenotyping screens will be granted based on scientific excellence and supports the development and in-depth characterisation of mouse models for investigating gene function and human pathophysiology. INFRAFRONTIER will provide open access to all characterised disease models and phenotyping data.