INFRAFRONTIER2020 Project
Trans-national Access call - November 2017

Precision mammalian model development
Call information and application form

Context and aim of the call

INFRAFRONTIER is the European Research Infrastructure for phenotyping, archiving and distribution of model mammalian genomes. The INFRAFRONTIER Research Infrastructure provides access to first-class tools and data for biomedical research, and thereby contributes to improving the understanding of gene function in human health and disease using the mouse model. The core services of INFRAFRONTIER comprise the systemic phenotyping of mouse mutants in the participating mouse clinics, and the archiving and distribution of mouse mutant lines by the European Mouse Mutant Archive (EMMA) (www.infrafrontier.eu).

Main objective of this INFRAFRONTIER open call is to facilitate access for the wider biomedical research community to the unique infrastructure and scientific expertise of the participating INFRAFRONTIER partners, to deliver novel mouse lines that will advance knowledge of human disease and will be of widespread use in biomedical science. Recent advances in genome editing technology will be used to develop new mouse models of human disease. INFRAFRONTIER will provide open access to all newly developed disease models through the European Mouse Mutant Archive (EMMA). Access to this free-of-charge-service will be granted on the basis of the applicant’s research plans and the potential impact of the proposed novel mouse line on the wider biomedical research community.

During the course of the INFRAFRONTIER2020 project a total of 3 Trans-national Access calls for the development of mammalian model development supporting a total of 28 projects will be published. Further calls supporting 10 mouse model and 6 rat model development projects will be released in 2018.

Proposal submission to proposals@infrafrontier.eu by 20 December 2017.
Proposal evaluation from 21 December 2017 to 15 February 2018.
The INFRAFRONTIER2020 project has received funding from the EU Research and Innovation programme Horizon 2020 (H2020-EU.1.4.1.1. Developing new world class research infrastructures)

Trans-national Access (TA) activity of the INFRAFRONTIER2020 project
Free of charge precision mammalian model development service / Access modalities

The EC Horizon 2020 funded INFRAFRONTIER2020 project (2017 – 2020) supports eligible customers with a free-of-charge mouse model development service implemented as a Trans-national Access activity supporting a total of 12 projects in this first call.

The access unit offered covers the production of a single F1 genome-edited mouse line (at least one individual of a F1 genome edited mouse line will be provided).

The model development service involves project design including prediction of off-target sites, preparation of sgRNA’s and Cas9 mRNA/protein, and injection into zygotes to generate F0 founder mutant animals (C57BL/6N or C57BL/6J genetic background preferred). Selected F0 animals will be bred to germ line to produce F1 genome edited animals. Possible allele types that can be generated are indels, exon deletions (< 10kb) and point mutation insertions. Newly developed mouse models will be made available to selected applicants within an average of 12 months following provision of all required information to start the mouse production.

The generated mouse models will be made available to the scientific community. An optional grace period of up to 1 year for mouse resources may apply, with immediate release of mouse resources after expiry of the grace period. Mouse mutant lines will be deposited into the INFRAFRONTIER/EMMA repository for subsequent use by the scientific community. Newly developed mouse models will be owned by the production centres and will be distributed by the INFRAFRONTIER/EMMA repository using their institutional MTAs.

Costs

The access to the INFRAFRONTIER2020 model development service is free of charge. However, the shipment cost of the newly developed mouse models must be borne by the applicants.

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