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InfrafrontierGR/Phenotypos Research Infrastructure
Trans-regional Phenotyping Call - June 2020

Τhe InfrafrontierGR/Phenotypos project ( supports eligible researchers with free-of-charge mouse phenotyping services implemented as a Trans-regional Access activity. A total of four (4) projects will be supported through this Call, one (1) project per assay type (i.e. 1 live imaging project, 1 microCT project, 1 blood analysis or cell sorting project, 1 histopathology project). Free access will be granted on the basis of scientific excellence and supports the development and in-depth characterization of mouse models to investigate gene function and human pathophysiology.

The services offered will be run by BSRC Fleming’s InfrafrontierGR Units and may involve one of the following assays:

1) Live imaging assays (Fleming mouse facilities)
- Colonoscopy assay for colitis/colorectal cancer mouse model assessment
- X-rays and bone density assays for osteoporosis/arthritis mouse model assessment
- Luminol bioluminescence imaging assay for acute inflammation assessment (arthritis, enteritis)
- Lucigenin bioluminescence imaging for chronic inflammation assessment (arthritis, enteritis)

2) Micro CT imaging assays
- Trabecular mouse femur analysis and 3D imaging
- Cortical mouse femur analysis and 3D imaging
- Calcaneus mouse bone analysis and 3D imaging

3) Blood Analysis assays and Cell sorting
- Complete Mouse Blood Count
- Biochemical analysis of mouse serum/plasma
- Basic Immunophenotyping of mouse blood
- Cell Sorting

4) Histology assays
- Standard mouse tissue processing for FFPE block generation
- Histology slide preparation
- Automated Hematoxylin/Eosin (H/E) slide staining and covering
- Multiple histochemical staining’s (Masson Trichrome, Luxol Fast Blue, Periodic Acid Schiff)

A collaboration agreement will be established between the selected applicants and BSRC Fleming.
All assays will be performed based on InfrafrontierGR Standard Operating Procedures.

Results will be made available to selected applicants within a maximum of 6 months following the provision of all required information and material.

The analyzed mouse models and the generated phenotyping data will be made available to the scientific community. Upon request, an optional grace period of up to 1 year for phenotyping data may apply, with the immediate release of data after the expiry of the grace period.

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