top of page

Trans-national Access call - April 2018

- The EC Horizon 2020 funded INFRAFRONTIER2020 project (2017 – 2020) supports eligible customers with a free-of-charge rat model development service implemented as a Trans-national Access activity supporting a total of 6 projects in this call. ​The access unit offered covers the production of a single F1 genome-edited rat line (at least one individual of a F1 genome edited rat line will be provided).

- The model development service using genome editing involves project design, preparation of sgRNAs and Cas9 mRNA/protein, and injection into zygotes to generate F0 founder mutant animals (Sprague Dawley genetic background).

- Selected F0 animals (very often mosaic) will be bred to germ line to produce F1 genome edited animals. Possible allele types that can be generated are indels, exon deletions (< 10kb) and point mutation insertions.

- Newly developed rat models will be made available to selected applicants within an average of 12 months following provision of all required information to start the rat production.

- The generated rat models will be made available to the scientific community. An optional grace period of up to 1 year for rat models may apply, with immediate release of rat resources after expiry of the grace period. Rat mutant lines will be deposited into the INFRAFRONTIER/EMMA repository for subsequent use by the scientific community. Newly developed rat models will be owned by the production centres and will be distributed by the INFRAFRONTIER/EMMA repository using their institutional MTAs.

bottom of page